Black Mamba appears in the Ultimate Universe as a member of the Serpent Squad, a group of female mercenaries. After having the Serpent Crown stolen from them, they battle the Fantastic Four. Black Mamba was knocked out of the fight by the Thing early on, and was taken into custody. After the Thing called the Serpent Squad "freaks" in passing, Black Mamba retaliated in anger by saying he would die alone because he is so sickening. Black Mamba's Ultimate design closely resembles her Earth-616 design, but it is unknown if she commands the darkforce in this alternate universe. Oddly enough, her real name seems to have been changed to "Janis."

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